Abstract
In vitro anticancer screening of different isothiocyanates (ITCs) [Allyl isothiocyanate (AITC), Phenylethyl isothiocyanate (PEITC) and Sulphoraphane (SUL)], derived from naturally occurring Eruca sativa seed oil (SO) against HepG2 human liver carcinoma cell line has been carried out using MTT, TBE and SRB bioassays. Combination of ITCs, in ratio (1:1:1) exhibited maximum percentage inhibition of 97.12%, 96.34% and 96.56% by MTT, TBE and SRB respectively against reference drug doxorubicin at concentration 50 µM. Percent inhibition has been found to be more pronounced in isothiocyanates combination compared to its natural source E. sativa seed oil.
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