Abstract
The proposed device, for massive DNA-based screening in limited-resource settings, comprises a centrifugal platform to perform isothermal solid-phase amplification in microarray format and a digital versatile disc drive to read the results.
Highlights
The development of DNA biosensors is related to the adequate selection and integration of support, probes, assay format, and transduction phenomena that are needed to perform and detect a biorecognition
The uidic valves were designed for use in the 0–1500 rpm range to allow for easy automation within a standard digital versatile discs (DVDs) drive
This study is aligned with recent investigations that are focused on merging optical discs and centrifugal micro uidics to create a new step towards increased automatization, reduced sample consumption and low cost diagnostics
Summary
The development of DNA biosensors is related to the adequate selection and integration of support, probes, assay format, and transduction phenomena that are needed to perform and detect a biorecognition. Plastic polymers, as analytical platforms, offer the advantages of being transformed and cheaply into devices that combine operations of sample treatment, uid management, and detection.[1] in some cases, the proposed platforms are not useful for real applications because the systems for uid management and signal detection are not adaptable to a wide range of scenarios. Several research groups and companies have been working on the development of biosensors based on the use of compact discs or ‘lab-on-a-CD’ systems. There are two main approaches depending on the nature of the disc used.[2] The rst one involves the use of plastic substrates that are several mm thick with
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