Abstract
// Xiaolong Shi 1 , Haiyan Zhao 1 , Xin Li 2 and Tao Song 3, 4 1 Key Laboratory of Image Information Processing and Intelligent Control, School of Automation, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China 2 Department of Gynecology 2, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China 3 College of Computer and Communication Engineering, China University of Petroleum, Qingdao 266580, Shandong, China 4 Departamento de Inteligencia Artificial, Universidad Politécnica de Madrid, Campus de Montegancedo, Boadilla del Monte 28660, Madrid, Spain Correspondence to: Tao Song, email: tsong@upc.edu.cn , t.song@upm.es Keywords: DNA nanotechnology; DNA origami; DNA nanotube; drug cargo Received: October 09, 2017 Accepted: November 13, 2017 Published: January 04, 2018 ABSTRACT DNA Nanostructures assembled from artificial single stranded DNA provided an engineering method to fabricate highly biocompatible spatial objects, which have potentials applications as drug delivery vehicles and templates for the patterning of biological molecules as biosensor for active hepatic targeting drug delivery. Most DNA nanostructures are constructed by annealing process, which means some thermal sensitive drugs and biosensors made from peptides or proteins in the structure will be denatured during fabrication. Fabrication of DNA nanostructure under isothermal condition remains challenging. Herein we report a simple and cost-effective method to form DNA nanotubes in the presence of urea with only two single-stranded tiles (SST) at room temperature. The constructed DNA nanotubes were observed and analyzed by atomic force microscopy (AFM). Experimental results show the feasibility and stability of our method. This method avoids traditional annealing procedure and will provide possibility to further assembly nano-scale structures in vivo .
Highlights
DNA, known as genetic materials for replication and storage of information in biology, is an ideal material for building up nanostructures due to the precisely predicted behavior according to the Watson– Crick base pairing rules
When DNA nanotubes are used as templates for patterning materials, it is desirable to form the nanostructure under isothermal conditions especially at room temperature, for some functional molecules such as proteins are thermally sensitive and will denature at high temperature
The two strands S1 and VS1 were mixed with a final concentration of 1 uM per strand in assembly buffer 1×TAE (20 mM Mg2+, 20 mM Tris with pH 7.6, 2 mM EDTA) containing 6M urea, and the mixture was subjected to incubation process at room temperature (21°C) for a time period
Summary
DNA, known as genetic materials for replication and storage of information in biology, is an ideal material for building up nanostructures due to the precisely predicted behavior according to the Watson– Crick base pairing rules. Several strategies have been used to construct DNA nanotubes These methods include DNA origami [11,12,13,14], DNA tile self-assembly [1, 3, 15,16,17,18] and SST (single-stranded tile) [7, 10, 19, 20]. These approaches use thermal annealing protocol in which component strands were mixed in a magnesium-containing buffer followed by annealing from high temperature (e.g., ~95°C) to room temperature. When DNA nanotubes are used as templates for patterning materials, it is desirable to form the nanostructure under isothermal conditions especially at room temperature, for some functional molecules such as proteins are thermally sensitive and will denature at high temperature (streptavidin, 75°C)
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