Isotherapic of Culex on the biological cycle of the mosquito Culex sp

Abstract

Introduction: Culex is an insect of the family Culicidae. It occurs in tropical and subtropical regions. It is known as the domestic mosquito. Their larvae develop in stagnant and dirty water, with plenty of organic matter. Some species of the insect such as Culex quinquefasciatus transmit the worms (helminths) called Wuchereria bancrofti filariasis or Elephantiasis.
 
 Aims: Thus, the objective of this work was to evaluate the effect of isotherapic of Culex on the biological cycle of this insect.
 
 Material and Method: The experiment was conducted at the Laboratory of Plant Physiology and Homeopathy at the State University of Maringa in the period from March 26 to December 31, 2007. Fifteen larvae of Culex sp. the same stage of development (Stage I) were used in the experiment with Isopathy of Culex sp. Was added 200 microliters of the dilutions of isotherapic 3, 6, 9, 12, 18, 24 and 30cH in each flask containing 30 mL of water and 15 larvae of Culex sp. The control consisted of 30 mL of water and 200 mL of 5% alcohol. The flasks were covered by tissue "toule"-type to prevent the dispersal of adult insects. The dilutions were produced according to the Brazilian Homeopathic Pharmacopoeia (1987) [1]. Were determined: average number of larvae, pupae and mosquitoes during the cycle of the insect.
 
 Results and Discussion: The presence of Culex isotherapic in the growth solution presented complex responses. Some dilution caused positive responses (3, 6, 9, 12 and 18cH) in the average number of larvae (Figure 1). Dilution 18cH apparently caused a protective effect, and somehow minimized the negative influence of the environment, or protect the larvae from unfavorable abiotic conditions. Dilutions 6, 18 and 24 cH, instead, caused adverse effect on larval and pupa survival, but positive when we consider the objective of this work. There is an interesting phenomenon when evaluating the biological responses in living according to the dilution used. The alternation of rises and falls in the physiological variables as a function of dilution were observed by several authors [2-5]. It behavior is not explained by science, but it is believed to be related to the rhythmic nature movement [6,7] and also with the law of similarity occurring between dilution and the organism that receives it. Thus, the physiological function of the dilutions in the same drug are often cyclical and not linear.
 
 Conclusion: The results of this experiment suggest that some dilution may be used in studies to control the mosquito Culex sp.