Abstract
Together with 2–5A synthetase and ribonuclease L, 2–5A phosphodiesterase belongs to the 2–5A system, which plays an important role in the action of interferon. Analytical capillary isotachophoresis was used for the determination of 2–5A phosphodiesterease activity. Enzyme assay was optimized using snake venom phosphodiesterase as a source of 2–5A phosphodiesterase activity. The 2–5A trimer core was used as a substrate. Enzyme activity was determined in time- and concentration-dependent reactions. In addition, 2–5A phosphodiesterase activity was determined in lysates of mononuclear blood cells.
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