Abstract
The isotachophoretic separation of several cations important in plant nutrition is described. The ion K + was used as the leading electrolyte, acetic acid as the counter ion and N-(2-acetamido)iminodiacetic acid the co-counter ion in case I, and N,N-bis(2-hydroxyethyl)glycine as the counter ion in case II. Tris(hydroxymethyl)aminomethane was used as the terminating electrolyte. Operating system is suitable for the separation and determination of Na +, Mg 2+, Ca 2+ and Li +, whilst system II is suitable for that of ▪, Na +, Mg 2+, Li +, Ca 2+, Mn 2+. The influence of the co-counter ion concentration and that of the pH of the leading electrolyte on the relative step heights of cations in system I was investigated. In system II the changes in concentrations of ▪, Na +, Mg 2+ and Ca 2+ in a nutritive solution due to the presence of 7-day-old winter wheat plants with time were observed.
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