Abstract
BackgroundIn this report, the isorhamnetin 3-o-robinobioside and its original extract, the ethyl acetate extract, from Nitraria retusa leaves, were evaluated for their ability to induce antioxidant and antigenotoxic effects in human chronic myelogenous leukemia cell line.MethodsNitraria retusa products properties were carried out by firstly evaluating their effects against lipid peroxidation induced by H2O2, using the thiobarbituric acid reactive substances species (TBARS) assay, and proceeding to the assay of cellular antioxidant activity, then doing the comet assay.ResultsThe isorhamnetin 3-o-robinobioside showed a protective effect against lipid peroxidation induced by H2O2. The same natural compound and ethyl acetate extract inhibited oxidation induced by 2,2′-azobis (2-amidinopropane) dihydrochloride in human chronic myelogenous leukemia cells with respectively 50% inhibitory concentration values of 0.225 mg/ml and 0.31 mg/ml, reflecting a significant antioxidant potential. The same two products inhibited the genotoxicity induced by hydroxyl radicals in the same human cell line (by 77.77% at a concentration of 800 μg/ml and by 80.55% at a concentration of 1000 μg/ml respectively).ConclusionsThe isorhamnetin 3- o-robinobioside and its original extract, the ethyl acetate extract, from Nitraria retusa leaves, have a great antioxidant and antigenotoxic potential on human chronic myelogenous leukemia cell line K562.
Highlights
In this report, the isorhamnetin 3-o-robinobioside and its original extract, the ethyl acetate extract, from Nitraria retusa leaves, were evaluated for their ability to induce antioxidant and antigenotoxic effects in human chronic myelogenous leukemia cell line
The structure of compound 1, purified from EA extract, was established and the compound was identified as a flavonol, isorhamnetin3-o-robinobioside (I3-O-Rob) (Figure 1)
Retusa products on lipid peroxidation induced by H2O2 The reaction of MDA with thiobarbituric acid (TBA) has been widely adopted as a sensitive assay method for lipid peroxidation
Summary
The isorhamnetin 3-o-robinobioside and its original extract, the ethyl acetate extract, from Nitraria retusa leaves, were evaluated for their ability to induce antioxidant and antigenotoxic effects in human chronic myelogenous leukemia cell line. Environmental mutagens and carcinogens are instrumental in initiation, promotion, and progression of several kinds of cancers. The exposure to these xenobiotics is often unavoidable and creates a great risk to human health. The leaves serve as supplement for tea and are used as poultice. The ashes of this species have the ability to remove fluids of infected wounds. Fresh leaves of Nitraria retusa decoction is used in Morocco in case of poisoning, upset stomach, ulcers, gastritis, enteritis, heartburn, colitis and colonic abdominal pain [10]
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