Isoproturon-Resistant Littleseed Canarygrass (Phalaris minor) and its Response to Alternate Herbicides1

Abstract

Littleseed canarygrass, a troublesome wheat weed, has developed resistance against isoproturon in India. Laboratory, pot, and field experiments were conducted to quantify the level of isoproturon resistance in various littleseed canarygrass biotypes and to evaluate the response of isoproturon-resistant littleseed canarygrass to alternate herbicides. For quantification of resistance, 10 littleseed canarygrass biotypes were collected from fields with a varied history of cropping and herbicide use. Pot studies indicated that 6 out of the 10 biotypes tested were resistant to isoproturon. The resistant (R) biotypes had 50% growth reduction values, 5 to 10 times greater than that of the most susceptible biotype. The R biotypes were from areas where isoproturon had been used continuously for more than 10 yr in wheat under a continuous rice–wheat cropping system. The isoproturon resistance was not detected where crops and herbicides were rotated. Under field conditions, postemergence isoproturon at 1,000 g/ha 32 d after sowing failed to control littleseed canarygrass and as a result wheat grain yield decreased by 65% compared with the weed-free control. The herbicides observed to be effective in wheat against isoproturon-resistant littleseed canarygrass were CGA 184927 (50 to 60 g ai/ha), fenoxaprop-p-ethyl (100 to 120 g/ha), ICIA 0604 (350 g ai/ha), and MON 37503 (25 g ai/ha). The use of these herbicides increased the grain yield by 200% or more when compared with the weedy check. Among the herbicides tested, MON 37503 at 25 g ai/ha was the best because of complex weed flora control and produced wheat yield similar to that of the weed-free control. Based on this study, the absence of resistance when herbicides and crops were rotated indicates that growers should follow herbicide and crop rotation in order to avoid development of herbicide resistance in the near future. The integration of this system (crop and herbicide rotation) with cultural and mechanical means of weed control could be further targeted as a long-term strategy for resistance management.Nomenclature: CGA 184927 (proposed common name, clodinafop), 2-propynyl-(R)-2-[4-(5-chloro-3-fluoro-2-pyridyloxy)phenoxy]propionate; fenoxaprop-p-ethyl; ICIA 0604 (proposed common name, tralkoxydim), 2-[1-(ethoxyimino)propyl]-3-hydroxy-5-(2,4,6-trimethyl phenyl)cyclohex-2-enone; MON 37503 (proposed common name, sulfosulfuron), 1-(2-ethylsulfonylimidazol[1,2-a]pyridin-3-ylsulfonyl)-3-(4,6-dimethoxypyrimidin-2-yl)urea; littleseed canarygrass, Phalaris minor Retz #3 PHAMI; rice, Oryza sativa L.; wheat, Triticum aestivum L.Additional index words: Biotype, competition, cross-resistance, germination, herbicide resistance.Abbreviations: CRD, completely randomized design; DAS, days after sowing; GR50, 50% growth reduction; R, resistant; RI, resistance index; S, susceptible.