Abstract
Lignin is formed in the cell wall of the downy mildew-infected Japanese radish root and peroxidase activity increases in the neibouring tissues. Attempts were made to study the functional role of this increased peroxidase activity in lignification in diseased tissues. The crude peroxidase prepared from the healthy tissue with ammonium sulfate precipitation and isoelectric point precipitation was fractionated into 11 fractions by cellulose column chromatography. The activity of the crude enzyme preparation increased gradually in response to the infection, reached a maximum 9 days after inoculation and then decreased. Elution profiles of peroxidase extracted from the root 9 days after inoculation showed that activities in acidic and neutral fractions apparently decreased while those of basic fractions increased. Polyacrylamide gel electrofocusing of the crude peroxidase preparation showed a decrease in activity of acidic isoperoxidases and an increase in activity of a part of basic and neutral isoperoxidases in the diseased tissue.
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