Abstract

Secretory cells specialized for monoterpene biosynthesis via the deoxyxylulose-5-phosphate pathway for terpenoid biosynthesis were isolated from peppermint ( Mentha piperita) leaves and used to identify the terminal metabolite of this novel pathway. Incorporation of both [ 13C]- and [ 14C]pyruvic acid into monoterpenes in the presence of 2-(dimethylamino)ethyl diphosphate, an inhibitor of isopentenyl diphosphate isomerase, results in accumulation of isopentenyl diphosphate, and not dimethylallyl diphosphate.

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