Isoliquiritigenin alleviated the Ang II-induced hypertensive renal injury through suppressing inflammation cytokines and oxidative stress-induced apoptosis via Nrf2 and NF-κB pathways

Abstract

PurposeHypertensive renal injury plays important role in the pathogenesis of end-stage nephropathy and the need for dialysis. Isoliquiritigenin (ISL) is a natural compound with antioxidant and anti-inflammatory activities. In this study, the protective effects of ISL on Angiotensin II (Ang II)- induced apoptosis, inflammation and extracellular matrix production in HK-2 cells were observed and its mechanisms were elucidated. MethodsCell survival was determined with MTT assay. Cell cycle and apoptosis was assessed with flow cytometric analysis. The production of cytokines including IL-1β and TNF-α were evaluated with Elisa. Western blotting assay was used to determine protein levels of apoptosis related signaling, oxidative stress, NF-κB and ECM related molecules. mRNA levels of fibronectin and collagen Ⅳ were detected by RT-qPCR. ResultsAng II significantly inhibited cell survival, induced cell cycle arrest and enhanced cell apoptosis. However, the above effects were markedly alleviated by ISL treatment in a dose-dependent manner. In addition, Ang II significantly induced oxidative stress and NF-κB signaling activation, as well as inflammatory cytokines release. In contrast, these effects were remarkably reversed by ISL via regulation of Nrf2. Notably, Ang II also triggered generation of extracellular matrix, including fibronectin and collagen Ⅳ, which was abolished upon ISL treatment. ConclusionsTaken together, ISL alleviated the Ang II-induced hypertensive renal injury through suppressing inflammation cytokines, excessive deposition of extracellular matrix and oxidative stress-induced apoptosis via Nrf2 and NF-κB pathways. Our findings provided the evidences for exploring the possible mechanism of hypertensive renal injury pathogenesis and identifying novel therapeutic targets.