Abstract
In an effort to minimize the risks for scientists exposure to the infectious environment and avoiding infectious incident, high-level bio-safety laboratories are designed and constructed to improve experimental safety by preventing laboratory infectious waste causing harm to people or the environment. The possibility of high-risk pathogens spread to the public environment with flow of people and materials and water and air is strictly controlled by improvement physical protection to reduce human infection rates and incidence of environment contamination to zero. The air flow safety, including interior mechanical ventilations and the suction and exhaust process in large working process equipments, is ensured by installing air conditioning system, air filter system, one working and one on standby extraction blowers, constant or variable air volume damper in pipes, automation and monitoring system, and power supply system (including dual power supply system and online emergency power supply) to maintain suitable directional negative filtering air flow with constant temperature and humidity working conditions for the operators. The materials flow safety, including experimental materials, laboratory animals and laboratory infectious waste, is mastered by installing transfer system (including delivery window and double port transfer exchange system) and sterilization system (double-doored autoclave). The liquid flow safety, including launching and softened water, is controlled by reverse osmosis disinfection system and independent sewage discharge system (including high temperature and high pressure sterilizer and chemical disinfection tank). The people flow safety, including walking around in the laboratory, carrying out experimental procedures, changing protective clothing, and physiological activity such as respiration, is protected by primary barriers (including biological safety cabinet and negative-pressure isolator), secondary barriers (building envelope and facility construction), third barriers (personal protective equipment, PPE), communication systems between the lab and outside, visual monitoring devices and alarm system.
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