Abstract

We developed eight new polymorphic microsatellite markers for Acacia montana, a bushy shrub from south-eastern Australia used in revegetation projects. Initially, primers were designed for 32 loci identified from 454 shotgun sequencing. Eight loci amplified successfully and were polymorphic when screened on 40 individuals, with observed heterozygosity ranging from 0.250 to 0.789 and the number of alleles per locus from 4 to 13. These loci will be useful in understanding the genetic variation and structure of A. montana in its current fragmented range.

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