Abstract
Abstract:
 The aerial part of Ephedra foliata Family Ephedraceae have long been used in traditional medicine and now Ephedra species have medicinal, ecological, and commercial value. The variety of pharmacological actions of this plant is due to its chemical constituents. Ephedrine and related alkaloids; are the newly potential medicinal value of Ephedra supplements for weight loss or performance improvement. Other pharmacological actions like antibacterial and antifungal effects of the phenolic acid compounds, the immunosuppressive action of the polysaccharides, and the antitumor action of flavonoids. The genus of this plant wildly distributed throughout asia, america, europe, and north africa. Aim of study: this study is to screen the phytochemical constituents due to the importance of pharmacological actions of this plant.
 Methods: the aerial part of E. foliata was macerated in 80% ethanol for 9 days and fractionated by n-hexane, chloroform, ethyl acetate, and n-butanol. The n-hexane, chloroform, n-butanol fractions, and isolated compounds were analyzed by GC-MS, TLC, UPLC ESI MS/MS.
 Results and discussion: The various chromatographic and spectroscopic results indicate the presence of a different type of phytochemicals like ephedrine, 6hka, vicenin 2 and quercetin 3-sophoroside-7-rhamnoside.
Highlights
The aerial part of Ephedra foliata Family Ephedraceae have long been used in traditional medicine and Ephedra species have medicinal, ecological, and commercial value
For at least five thousand years, ephedra plants have been used in traditional medicines in which dry stems are used for symptoms derived from the common cold, flu, asthma, bronchitis, nasal congestion and hay fever[1]
The GC MS result showed the presence of ephedrine in chloroform fraction at peak 3 (Figure 8 and Table 3) isolated AS2 investigate by GC MS as ephedrine according to its retention time, molecular weight [165] and base peak [58] depending on NIST database (Figure 9,10,Table 4)
Summary
March – June 2018 from Tikrit province, Iraq. This plant was authenticated by Dr khansaa rasheed / Iraq Natural History Research Center and Museum Plant and Environment Department / University of Baghdad. Purification of crude alkaloidal extract: The chloroform fraction was acidified by adding hydrochloric acid (5%). This solution was placed in a separatory funnel and partitioned with equal volume of chloroform (four times). The chloroform ilayer iwas iseparated, dried with anhydrous sodium sulfate, filtered and evaporated under reduced pressure tested with Dragendorff and Mayer’s reagents[24]. Electrospray ionization mass spectrometry in negative and positive ions acquisition mode is performed in XEVO TQD triple quadruple instruments. The sample solution (100 μg / ml) is prepared using high-performance liquid chromotography (HPLC) analytical grade methanol, the filtrate uses a membrane disk filter (0.2 μm), subjected to LC / ESI / MS. Peaks and spectra are processed using Maslynx (4.1) software and are tentatively identified by comparing their retention times and masses spectra with the reported data [27]
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Schedule a callMore From: Iraqi Journal of Pharmaceutical Sciences ( P-ISSN: 1683 - 3597 , E-ISSN : 2521 - 3512)
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