ISOLATION, SREENING AND OPTIMIZATION OF SUBMERGED CULTURE CONDITIONS FOR MYCELIAL BIOMASS PRODUCTION WITH ENHANCED ANTIBACTERIAL ACTIVITY OF THE FUNGUS MYROTHECIUM SPP. MRP001 AGAINST MULTI DRUG RESISTANT PATHOGENS

Abstract

Fungi are interestingly; produce the bioactive natural products thus being screened for the production of valuable antibacterial product. In the present study the fungus was isolated from soil and identified as Myrothecium spp. MRP001 based on morphological characteristics and it was reinforced by 16S rRNA gene sequence analysis. The submerged culture conditions and nutritional requirements for the production of mycelial biomass with improved antibacterial activity were studied in shaker flask culture using OFAT (one factor at time) in six different media, After 120 h of growth, Tryptone Soya Broth (TSB) and Potato Dextrose Broth (PDB) shows maximum spore account (2×108 CFU/ml) of Myrothecium spp MRP001. Among the all different culture media, the maximum biomass production of 2.14 g/L and 2.23 g/L were observed in Tryptone Soya Broth (TSB) and Potato dextrose broth (PDB) respectively, over a period of 120 h of incubation at pH of 6.5 in 25-30 °C in Myrothecium spp. Strain MRP001. Among the carbon sources, glucose supplemented basal medium showed an effective biomass production of 2.24 g/L. whereas in nitrogen sources, yeast extract had significant biomass production of 2.12 g/L. The optimized ethyl acetate extract of cultural filtrate showed a maximum inhibition zone of 28.4 mm and 26.3 mm against multidrug resistant P. aeruginosa strain 1 and S. aureus strain 5, respectively. These results are useful for further investigation of the fungus in the future for microbial disease treatment for multi drug resistant human pathogens.