Abstract
Pharmacognosy Research,2022,14,1,30-34.DOI:10.5530/pres.14.1.6Published:December 2021Type:Original ArticleAuthors:Subashini Subramanian, and Shakila Ramachandran Author(s) affiliations:Subashini Subramanian, Shakila Ramachandran* Department of Chemistry, Siddha Central Research Institute, Anna Hospital Campus, Arumbakkam, Chennai, Tamil Nadu, INDIA. Abstract:Background: Taxifolin (TA) is a flavonoid that has antioxidant, hepatoprotective, antialzheimer, anti-hyperglycemic, cardiovascular, anti-inflammatory, anti-psoriatic and antialzheimer properties. Taxifolin 3-O-rhamnoside (TAR), a glycoside of taxifolin, has antioxidant, anticonvulsant, anticancer, anti-inflammatory, and immunosuppressive effects. Objectives: The goal of this study is to separate TA and TAR from Smilax china Linn. rhizomes, as well as to develop and validate a technique for simultaneous measurement of TA and TAR. Materials and Methods: The hydro alcoholic extract of the rhizome of S. china yielded TA and TAR. HPLC system was fitted with a C18 column (shim-pack) of size 150 mm x 4.6 mm; 5μ, with suitable eluting mixture of methanol: water (90:10 v/v) at a flowing rate of 1 ml/min and at 254 nm wavelength for identification of peaks. Lab solution software was used to establish the quantification method for above-mentioned chemical compounds. Results: TAR and TA were separated using the proposed technique at Rt 2.917 and 3.924 min respectively. Over the range of 0.1-0.8 μg/ml, calibration curves were produced with a linear relationship R2 > 0.9941 and 0.9963, respectively. The relative standard deviation was less than 2%. The percentage recoveries were shown to be between 97 and 102.1. TA and TAR had detection limits of 0.156 and 0.077 μg/ml; quantification limits were 0.473 and 0.234 μg/ml respectively. The technique that was devised was simple, sensitive and specific. Conclusion: The newly designed RPHPLC technique has improved specificity, precision, and accuracy. The quality of S. china and other dietary supplements containing these two flavonoids may be successfully assessed by quantifying these two flavonoids and their glycosides. Keywords:3, 3’, 4’-pentahydroxy flavanone, 5, 7, Astilbin, Dihydroqucertin, Tuberculosis, Validation.View:PDF (505.31 KB) Full Text
Highlights
Smilax china Linn, a member of the Smilacaceae family, grows widely in tropical and temperate locations around the world, in East Asia.[1,2] This plant is a perennial climber with aculeate pore skin and paired tendrils that help with climbing
Plant extracts of the Smilax genus have antioxidant and pro-apoptotic properties.[6] (2R,3R) Hayashi and Ouchi was the first one to isolate taxifolin3-β-O-rhamnoside from the rhizome of Astilbe thunbergii.[7]. Astilbin is another name of (2R,3R)taxifolin-3-β-O-rhamnoside. It is present in other plants consisting of Dimorphandra mollis, Psychotria prumfolia, Senna obtusifolia, Tithonia diversifolia,[8] Heritiera littoralis,[9] Engelhardtia roxburghiana,[10,11] Smilacis glabrae,[12] Smilacis chinae,[13] Drimys brasiliensis,[14] Hymenaea courbaril,[15] Hymenaea stigonocarpa,[16] Pieris japonica,[17] previous research has suggested that astilbin has the potency to be used in both healthy supplements and medicine because of its various bioactivities, increasing liver injury immunological activity.[7]
NMR was used to analyze the spectral data, which was compared to taxifolin,[26] and taxifolin 3-O-rhamnoside.[27]
Summary
Smilax china Linn, a member of the Smilacaceae family, grows widely in tropical and temperate locations around the world, in East Asia.[1,2] This plant is a perennial climber with aculeate pore skin and paired tendrils that help with climbing. Plant extracts of the Smilax genus have antioxidant and pro-apoptotic properties.[6] (2R,3R) Hayashi and Ouchi was the first one to isolate taxifolin3-β-O-rhamnoside from the rhizome of Astilbe thunbergii.[7] Astilbin is another name of (2R,3R)taxifolin-3-β-O-rhamnoside It is present in other plants consisting of Dimorphandra mollis, Psychotria prumfolia, Senna obtusifolia, Tithonia diversifolia,[8] Heritiera littoralis,[9] Engelhardtia roxburghiana,[10,11] Smilacis glabrae,[12] Smilacis chinae,[13] Drimys brasiliensis,[14] Hymenaea courbaril,[15] Hymenaea stigonocarpa,[16] Pieris japonica,[17] previous research has suggested that astilbin has the potency to be used in both healthy supplements and medicine because of its various bioactivities, increasing liver injury immunological activity.[7] Taxifolin ( known as 3,5,7,3,4’-pentahydroxy flavanone or dihydroquercetin) is a flavonoid and an integral ingredient of nutritional dietary supplements. Results: TAR and TA were sraenpgaeraotef d0.1u-s0i.n8gμtgh/me lp, rcoapliobsraetdiotneccuhrnviqeusewaetreRpt r2o.d9u1c7eadnwdit3h.9a2l4inemarinrerleastipoencsthivipelRy.
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