Isolation, Sequence Identification and Tissue Expression Distribution of Three Novel Porcine Genes-UBC1, TRA2A and SH21A

Abstract

Liu G.Y., Zhao, S.M., Song, X.L., Wang, J. and Gao, S.Z. 2008. Isolation, sequence identification and tissue expression distribution of three novel porcine genes-UBC1, TRA2A and SH21A. J. Appl. Anim. Res., 33:33–38. Ubiquitin-conjugating enzyme E2–25 kDa (UBC1), transformer-2 protein homolog TRA-2 alpha (TRA2A) and SH2 domain protein 1A (SH21A) genes are most important genes highly related or potentially related with the porcine production. The complete coding sequences of these three porcine genes were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved sequence information of the mouse or other mammals and referred highly homologous pig ESTs. Porcine UBC1 gene encodes a protein of 200 amino acids which contains the conserved putative UBCc domain and has high homology with the UBC1 proteins of three species-human, mouse and bovine (100%). The porcine TRA2A gene encodes a protein of 279 amino acids that contains the conserved putative RNA recognition motif (RRM) domain and has high homology with the TRA2A proteins of two species-human (99%) and mouse (97%). The porcine SH21A gene encodes a protein of 128 amino acids that contains the conserved putative src homology 2 (SH2) domain and has high homology with the SH21A proteins of two species human (92%) and mouse (86%). The tissue expression analysis indicated that the swine UBC1, TRA2A and SH21A genes were expressed in different tissues including lung, fat, small intestine, large intestine, liver, muscle, spleen and kidney. Our experiment is the first to establish the primary foundation for further research on these three swine genes.