Abstract
The Can Gio Mangrove is a Biosphere Reserve of UNESCO since 2000 and it is also a well-known example of “mangrove afforestation and reforestation area’’. A total of 63 actinomycetes were isolated from 25 samples of 9 different sites in mangrove forest soil Can Gio, HoChiMinh city, Vietnam. Almost their colonies have round-shaped; milky, white clear and yellow, entire or loabate margin; diameter size of these colonies varied from 0.2 to 3.0 mm. Twenty-nine of 63 tested isolates could produce antimicrobial active metabolites inhibiting at least one of the tested pathogens and 9 isolates were selected for 16S rDNA sequencing. The result showed that genus Streptomyces (8 isolates) and other genera including 1 isolates were Gram-negative bacteria (Stenotrophomonas). The antimicrobial activity and the amplifying genes coding for polyketide synthetase (PKS) and nonribosomal peptide synthetase (NRPS) showed that 8 strains had broad-spectrum antimicrobial activity, mainly against gram-positive bacteria as Bacillus cereus and Staphylococcus aureus.
Highlights
UNESCO since 2000 and it is a well-known example of 7% of algae and 2% of protozoa. (Pallaa et al, 2018)
The antimicrobial activity and the amplifying genes coding for polyketide synthetase (PKS) and nonribosomal peptide synthetase (NRPS) showed that 8 strains had broad-spectrum antimicrobial activity, mainly against gram-positive bacteria as Bacillus cereus and Staphylococcus aureus
4oC for at least 2 h to allow the diffusion of crude CanGio mangrove forest
Summary
UNESCO since 2000 and it is a well-known example of 7% of algae and 2% of protozoa. (Pallaa et al, 2018). Actinomycetes are group of aerobic, branched, actinomycetes were isolated from 25 samples of 9 different unicellular Grampositive bacteria with high percentage sites in mangrove forest soil Can Gio, HoChiMinh city, Vietnam. Almost their colonies have round-shaped; milky, white clear and yellow, entire or loabate margin; diameter size of G+C (70%) in their geneticmaterial. Many researchers discovered novel actinobacteria from poorly explored mangrove habitats such as isolation of Asanoa iriomotensis (Tamura and Sakane, 2005), Nonomuraea maheshkhaliensis According to Anzai et al (2008) out of
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