Abstract
Cacao (Theobrama cacao) is one of the main plantation commodities in Indonesia which has an important role for the national economy. The low productivity of cacao plants in Indonesia is due to the condition of old plants, pests and diseases attacks which affect the quality of the fruit and decrease the plant’s productivity. The objective of this study was to isolate and identify of endophytic bacteria from stem, flower, leaf and fruit of T. cacao by using 16S rRNA gene as genetic marker. Twenty seven endophytic bacterial isolates were collected from local plantation in Yogyakarta area. From this study, 8 endophytic bacterial strains exhibited the higher PGP traits. The isolates produced Indole Acetic Acid level by 0.3 to 5.21 ppm/hour. All of isolates had nitrogen fixation activity but have not phosphate solubilization activity. Among them, isolates CSDT 4 and CGKBH 4 showed promising potential as PGP bacteria. Based on the 16S rRNA gene sequences, those bacterial strains were identified as Brevibacillus brevis (CSDT 4) and Pantoea sp. (CGKBH 4). We propose that the B. brevis and Pantoea sp. which is reported for the first time for their PGP potential in cacao, exerts its beneficial effects on cacao crop through combined of activities. The potential PGP bacteria from the Cacao plant was used to make a specific bio-fertilizer formula for the Cacao plant, because of the different needs and condition that every plant requires.
Highlights
Cacao (Theobroma cacao L.) is an annual plant with the humid tropical habitat
Isolation and purification of endophytic bacteria In this study, we found that cacao plant harbored an abundance of culturable endophytic bacteria, and varied between stems, leaves, and fruits
Preliminary characterization of these isolates indicated that studied parts of cacao plant contained both Gram negative and Gram-positive bacteria
Summary
Cacao (Theobroma cacao L.) is an annual plant with the humid tropical habitat. This plant is exported commodities that generate high economic income for Indonesia. The cacao are source of bioactive compounds that gives a wide variety of valuable food products and beneficial impact for human health. It can be used for livestock feeds. Drought is environment stress for cacao plants besides pests and diseases. In a study conducted by Prihastanti (2010), drought stress affects the chlorophyll content of cacao seedling leaves. Plants grown on 50% groundwater content have lower chlorophyll a and b content than those grown on 75% groundwater content and the productivity decreases
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biosaintifika: Journal of Biology & Biology Education
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
