Abstract
The isolation procedure for a new species of ribosomal RNA is described. The name 5 s ribosomal RNA is proposed for this component. It has been found to occur in ribosome preparations from many different organisms. Approximately one molecule of 5 s rRNA is associated with the ribosome. It has a chain length of 120 in ribosomes from Escherichia coli and 148 in ribosomes from Blastocladiella emersonii . The major base composition is similar to transfer RNA but no methylated bases can be detected. We have estimated that about 0.5 residue of pseudouridine occurs per molecule. Other physical and chemical properties are described.
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