Abstract
Initial isolation and characterization screens have been carried out on the Jamaican ball moss (Tillandsia recurvata L.) plant producing a novel complex gly-cosidic compound. This complex glycoside may be responsible for the promising anti-cancer and anti-inflammatory properties of this ball moss plant. A combination of supercritical fluid processing and chromatographic purification was used to produce the isolate. LC-MS analysis showed the presence of a compound with a molecular weight of ~800 and mass spectral (MS-MS and MS-MS-MS) data indicate that this compound may be a glycoside with a molecular mass of 1601.1 Da (negative ionization mode) and possibly contain up to two sialic acids, based on the doubly-charged state of the molecule at neutral pH conditions. 2D-TOCSY NMR spectrum lends evidence to the presence of at least one likely amide to aliphatic proton correlation, indicating the presence of a sialic acid moiety. The main structure of this therapeutic compound appears similar to a soyasaponin.
Highlights
Cancer is a disease of de-regulated cell proliferation and is the leading cause of death in Jamaica [1] and the third leading cause of death worldwide [2,3,4] with an annual projection of 26 million new cases by 2030 of which 17 million deaths will occur [5]
LC-MS analysis showed the presence of a compound with a molecular weight of ~800 and mass spectral (MS-MS and MS-MS-MS) data indicate that this compound may be a glycoside with a molecular mass of 1601.1 Da and possibly contain up to two sialic acids, based on the doublycharged state of the molecule at neutral pH conditions. 2D-Total Correlation Spectroscopy (TOCSY) NMR spectrum lends evidence to the presence of at least one likely amide to aliphatic proton correlation, indicating the presence of a sialic acid moiety
CO2 exposed to specific conditions (204 atm and 40 ̊C), behaved like a non-polar organic solvent and its selectivity was enhanced by the use of polar entrainers or co-solvents such as ethanol or methanol [15], While the solvation conditions to fractionate the active constituents can vary from 100% carbon dioxide (CO2) to 100% methanol (CH3OH), the optimum levels were found to be 70% carbon dioxide and 30% methanol
Summary
Cancer is a disease of de-regulated cell proliferation and is the leading cause of death in Jamaica [1] and the third leading cause of death worldwide [2,3,4] with an annual projection of 26 million new cases by 2030 of which 17 million deaths will occur [5]. Previous studies conducted on the Jamaican ball moss indicated that this plant exhibits anti-inflammatory and anti-cancer potential against several cancer cell lines including; ex. The search for treatment associated with fewer side effects against these deadly diseases is on-going and so this research undertook investigations to determine the active ingredient in ball moss possibly responsible for the plant’s anti-cancer and anti-inflammatory properties
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