Isolation, purification and osteoinduction differentiation of canine bone marrow mesenchymal stem cells: Feasibility ofin vitroisolation using Ficoll density gradient centrifugation

Fang Xie ,Jiu Jun Xu ,Ran Xiao ,Lei Cai ,Xiaolei Jin ,Li Teng ,Yuan-Ning Cao

doi:10.3969/j.issn.1673-8225.2010.06.001

Isolation, purification and osteoinduction differentiation of canine bone marrow mesenchymal stem cells: Feasibility ofin vitroisolation using Ficoll density gradient centrifugation

Fang Xie , Jiu Jun Xu + Show 5 more

https://doi.org/10.3969/j.issn.1673-8225.2010.06.001

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Journal: Journal of Clinical Rehabilitative Tissue Engineering Research

Publication Date: Feb 1, 2010

#Bone Marrow Mesenchymal Stem Cells #Percoll Density Gradient Centrifugation + Show 8 more

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Abstract

AbstractBACKGROUND:Classic isolation method of bone marrow mesenchymal stem cells (BMSCs) is Percoll density gradient centrifugation. Blood cell component was removed. However, this method is complicated. Preparation density was needed when isolating dog bone marrow. Moreover, centrifugation was fre

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