Isolation, Purification and Characterization of Oil Degrading Bacteria from Different Oil Cake Samples

Abstract

Oil cakes of mustard and linseed were collected from Rambagh, Allahabad and Mahewa Gate, Allahabad. Two samples of mustard and one sample of linseed cake were taken as a sample for project. Bacteria were grown on these oil cake samples. From it different cultures were isolated and each was separately grown. The pure cultures grown were tested for oil degrading property. Those cultures, which showed oil degrading property, were selected and isolated. The cultures were then identified using Gram staining technique. A series of biochemical tests were done for the cultures consuming oil. The tests include starch hydrolysis test, urease production test and catalase production test. DNA was then isolated from these oil degrading cultures.