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Abstract
The genomic DNA of bacteria is highly compacted in one or a few bodies known as nucleoids. In order to understand the overall configuration and physiological activities of the cyanobacterial nucleoid under various growth conditions and the role(s) of each nucleoid protein in clock function, thylakoid membrane-associated nucleoids from the Synechococcus elongatus (se) PCC 7942 strain were isolated and purified in presence of spermidine at low salt concentrations by sucrose density gradient centrifugation. The sedimentation rates, protein/DNA composition and microscopic appearances as well as variation in structural components of clock proteins from the isolated nucleoids were compared under identical conditions. Microscopic appearances of the nucleoids were consistent with the sedimentation profiles. The nucleoid structure in the wild type was more tightly compacted than that in the KaiABC mutant strain. Western immunoblot analyses revealed that the KaiC was associated with the nucleoid fraction whereas maximum KaiA was localized in the cytosolic fraction, supposedly in association with the translation machinery.
Highlights
The chromosomes in the eukaryotic nucleus are compacted into the nucleosomes with histone proteins as the basic unit
In bacteria there is no such nucleosome, but the DNA is organized into a nucleoid body with particular proteins in the cell, which has a highly organized architecture based on condensation and coiling of DNA [1]-[6]
Viable cells were measured with a viable colony plate counting method and the concentration of total proteins were measured by OD at 550 nm and visualized by Coomassie Brilliant Blue (CBB) staining
Summary
The chromosomes in the eukaryotic nucleus are compacted into the nucleosomes with histone proteins as the basic unit. In bacteria there is no such nucleosome, but the DNA is organized into a nucleoid body with particular proteins in the cell, which has a highly organized architecture based on condensation and coiling of DNA [1]-[6]. At present a clear picture of cyanobacterial nucleoid organization is far from being complete This may be due to insufficient documentation of the molecular structure and composition of cyanobacterium Synechococcus elongatus (se) PCC 7942 nucleoid and its circadian rhythm dependent variation [25]
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