Abstract
Although it was previously found that cyclodextrin glucanotransferase (CGTase) produces cyclodextrins (CDs) composed of six to seventeen glucopyranose units, the existence of CDs which have more than eighteen glucopyranose units has still not been confirmed. Cyclomaltooctadecaose (v-CD) and cyclomaltononadecaose (c > CD) are new large-ring CDs (LR-CDs) composed of eighteen and nineteen glucopyranose units, respectively. v- and ξ-CD were purified from the commercially available CD powder produced by CGTase, by a combination of HPLC and column chromatography. The molecular weights of v- and ξ-CD were determined by FAB-MS, and their cyclic structures were identified by 1H-NMR and 13C-NMR. The 13C-NMR chemical shifts of v- and ξ-CD were elucidated and compared with those of already confirmed LR-CDs, and their structures were predicted from the results.
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