Isolation, porification & maintenance of pure culture and growth of oyster mushroom (p. sajor caju) on difference solid media

Abstract

Experiments were conducted at Department of Plant Pathology, T.D.P.G. College, Jaunpur in the two consecutive year 2010-2011 and 2011-12.Edible mushroom Pleurotus sajor caju (Fr.)’ Singer to evaluate the Growth of P. sajor caju on difference solid media.The experiments were laid out in completely Randomized design. The pure culture of P. Sajor caju obtained from mushroom research lab department of Plant Pathology, N.D.U.A.T., Faizabad. Isolation & Porification done by standard method. Growth of P. Sajor caju tested on different non-synthetic & synthetic solid media. To find out the best suited medium for growth of the P. sajor caju under present study, The experiment consisted of two type of solid media viz. (a) Non-Synthetic (Potato dextrose agar medium, Oat meal Agar Medium and Corn Meal Agar Medium), (b) Synthetic (Richard’s Medium,Czapek’s (Dox) agar Medium and Brow’s Starch Agar Medium). The different constituents of media were dissolved in distilled water separately and for solidification 2% agar was added. In case of Potato dextrose, Oat meal, cornmeal extraction were taken after boiling them on a water bath. All the media were sterilized in the autoclave at 1.1 kg pressure/cm2 for 20 minutes. The petridishes were handled aseptically in a inoculation chamber using a sprit lamp flame. The medium in the plates was allowed to solidify before inoculation with P. Sajor caju. During both the years solid media of Non-Synthetic, Potato Dextrose Agar medium were observed significantly higher than other tested, medium. Czapek agar medium was also significantly superior than oat meal (7.4 cm of 7 days after inoculation). Lowest mycelial growth was observed on Brow’s agar medium.