Isolation, physiological characters and effectivity of bacterial isolates of root nodules from various plants on the growth of Vigna radiata L

Abstract

Mung bean (Vigna radiata L.) are legume plants that can establish symbiosis with Rhizobacteria to fix N2 from air. Research on isolation, physiological characters and effectiveness of bacteria isolates of root nodules from various plants on the growth of Vigna radiata L has been carried out.The objective of study was to get effective Rhizobacteria isolates which can be potentially used as biological fertilizer agents. Characterization of 16 isolates were carried out and all were included in the fast growing group. For physiological characters of 16 isolates showed that11 isolates were able to produce IAA, 7 isolates were able to solubilize phosphate, 13 isolates were able to produce siderophore, and 16 isolates had protease enzyme and catalase activities. Ten isolates that have growth support activities: AD (1), AD (2), AD (3), AD (4), AD (7), AD (10), LK (2), EKP (1), EKP (2 ), and KcKP (1) were tested for its effectiveness on Vigna radiata L. As a control plant without inoculation on soil media (K1) and plants without being inoculated in plants in soil and compost media (K2).The design used was Completely Randomized Design. The plants were harvested at the age of 55 days. The parameters which were observed were: height of plant, number of leaves, dry weight of canopy, roots, root nodules, total of plants, wet weight of pods, number of pods and chlorophyll content. Inoculation of isolate AD (7) had increased the growth of Vigna radiata L compared to control.