Isolation, phylogenetic analysis and antimicrobial activity of halophilic actinomycetes from different saline environments located near Çorum province

Abstract

The aim of this study was to isolate and characterize halophilic actinomycetes. Halophilic actinomycetes isolates were obtained from soil samples from Iskilip-Kekec, Tayrak, Yerlikoy and Sungurlu-Akpinar, Akcakoyunlu, Alibaba, Boncuk salterns located near Corum province with a dilution plate technique on four selective media with 3%, 10% and 15% NaCl. PCR amplification belonging to 16S rRNA gene region of the isolates was realized out with primers of 27F and 1525R. Phylogenetic trees were formed with the three different algorithms in MEGA 7.0 software. All fifteen microorganisms were isolated on four selective media with different salt proportions and based on 16S rRNA gene sequence analysis of 15 isolates, eleven Streptomyces isolates, two Nocardiopsis isolates, two Saccharopolyspora isolates were determined. 7 of the strains exhibited only antibacterial activity, 3 only antifungal activity, and 1 both antibacterial and antifungal activities In addition, NRPS, PKS-I ve PKS-II gene regions of isolates with antimicrobial activity were investigated with related primers. According to the results of the work done, of the 11 actinomycete isolates, 4 were found to have PKS-I, 5 were PKS-II and 8 had NRPS gene regions. When the results were evaluated, it was observed that the isolates contained more nonribozomal peptide synthetase (NRPS) gene clusters than type I-II polyketide synthase (PKS-I-II) gene clusters. In addition, it was determined that 1 isolate contain three gene clusters together. As a result, it was found that actinomycetes isolated from saline soils have the capacity to produce secondary metabolites.