Isolation, Phenotypic Identification and Antibiotic Resistance Profile of Bacterial Isolates from Intestinal Fluids of Local Minahasa Pigs, North Sulawesi, Indonesia

Abstract

The Minahasa local pig is unique because it is the oldest local pig that has spread to the Philippine islands. Minahasa local pigs have endemic characteristics because they are in the Wallacea zone. Research has been carried out to isolate bacteria from pig intestinal fluid and obtain an antibiotic response profile from pure bacterial isolates. Pig samples were obtained from two locations in North Sulawesi, namely in North Minahasa and North Minahasa. Intestinal fluids are taken immediately when the pig is slaughtered. The liquid was preserved in a sterile container and inoculated directly on the nutrient agar medium by the dilution method. The bacterial isolates obtained were pure cultured and then used for automatic phenotypic identification using Vitek 2 Compact. The results showed that pure culture isolates were obtained from intestinal fluids of local pigs in Minahasa, North Sulawesi, indicating that two isolates (S1 and S2) were Escherichia coli and S3 was Enterobacter aerogenes. Isolate S1 showed resistance to Ampicillin, while isolate S2 showed resistance to Tetracycline, furthermore isolate S3 showed resistance to Tetracycline, Furanes, and Trimethoprim/Sulfonamide. The results confirm that further research is needed to isolate and test the antibiotic resistance of bacteria from pig intestines in several locations and various stages of common local pigs.