Isolation, Optimization and Molecular Characterization of Novel Lipase Producing Lysinibacillus macroides FS1 from Oil Rich Sample for Biodiesel Production

Abstract

Lipase is one of the promising biocatalyst in field of Enzymatic Biodiesel production. Screening of lipase producing bacteria and its media optimization facilitates enhanced lipase production. Considering the advantages of enzymatic biodiesel production using lipases the objective of present work was to isolate lipase producing bacteria and its media optimization. Bacterial strain were isolated from oil rich sample showing maximum zone of hydrolysis on TBA Media was selected and identified as Lysinibacillus macroides FS1 by 16SrDNA gene sequence analysis. Lipase production was carried out in production media and lipase activity of 3.1U/ ml was assayed by titrimetric method. Various media parameters were optimized and maximal lipase activity of 16.75U/ml were observed at 48 hr of incubation in optimized media with Honge oil as inducer, Galactose as carbon source , Ammonium Chloride and Beef extract as source of nitrogen, pH 7 and 37ºC temperature with agitation speed of 120 rpm. Under optimized condition 5.4 fold increases in the lipase activity than innate activity was observed. 50.74% yield was observed with specific activity of 21.25U/mg after purification with 30% ammonium sulphate. Purified lipase was stable in presence of methanol at 37°C. Results obtained shows that Lipase from Lysinibacillus macroides FS1 was promising biocatalyst for Biodiesel production.