Isolation of vanadium-resistance endophytic bacterium PRE01 from Pteris vittata in stone coal smelting district and characterization for potential use in phytoremediation

Abstract

This study investigates the V-resistant endophytic bacteria isolated from V-accumulator Pteris vittata grown on stone coal smelting district. Among all the ten isolates, the strain PRE01 identified as Serratia marcescens ss marcescens by Biolog GEN III MicroPlate™ was screened out by ranking first in terms of heavy metal resistance and plant growth promoting traits. The S. marcescens PRE01 had strong V, Cr and Cd resistance especially for V up to 1500mg/L. In addition, it exhibited ACC deaminase activity, siderophore production and high indoleacetic acid production (60.14mg/L) and solubilizing P potential (336.41mg/L). For heavy metal detoxification tests, PRE01 could specifically assimilate 97.6%, 21.7% and 6.6% of Cd(II), Cr(VI) and V(V) within 72h incubation. Despite the poor absorption of the two anions, most V(V) and Cr(VI) were detoxified and reduced to lower valence states by the strain. Furthermore, the isolate had the potential to facilitate the metals uptake of their hosts by changing heavy metal speciation. Our research may open up further scope of utilizing the endophyte for enhancing phytoextraction of vanadium industry contaminated soils.