Abstract

Vacuoles were isolated from the upper epidermis of petals of Petunia hybrida line R27 using three different procedures. Utilizing the vacuolar localization of anthocyanin, vacuolar prep­arations obtained through polybase treatment, osmotic shock and mechanical forces were com­pared on purity, yield and stability. The comparison indicated that application of the polybase procedure results in the best vacuolar preparations. Vacuoles could be obtained which main­tained their acidic pH and retained their anthocyanin content.

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