Isolation of Ureolytic Bacteria of Soft Coral and Their Potential in Microbially Induced Calcite Precipitation (MICP)

Abstract

Microbial-induced CaCO3 precipitation (MICP) via urea hydrolysis (ureolysis). Application of MICP is performed either by augmenting the site with ureolytic bacteria or by stimulating indigenous ureolytic bacteria. Alternative approaches to promote in situ MICP in soils biostimulation. Biostimulation encourages indigenous urea-hydrolysis bacteria by providing appropriate enrichment and precipitation media; it relies on the natural ubiquity of ureolytic soil bacteria. This study aims to obtain potential bacteria for calcium carbonate precipitation. The research methods used were (1) isolation of indigenous bacteria from soft corals and selection of potential bacteria for calcium carbonate precipitation; (2) morphological identification of selected isolates; (3) characterization of bacterial activity. This research has successfully grown 5 indigenous bacterial isolates from soft corals. One isolate, MICP-36, was able to grow on Marine Agar containing 25 g/L urea. The results MICP-36, was Gram positive and had a prosthetic cell shape. This isolate can induce calcium carbonate deposits of 0.083 g / mL. The test survival of bacteria in concrete showed that MICP-36 was able to live until the 28th day.