Isolation of tumor disturbing factor on the proliferation of tumor cells in human serum

Abstract

The classified sediment with ethanol from sera of nude mice and humans showed a disturbing effect on L1210 cells in vitro and a lifesaving effect on L1210 cell-bearing mice in vivo . This factor was purified more than 2300-fold to a specific activity of approximately 1 × 10 5 U/mg by ethanol classified precipitation, Sephadex G-200 gel filtration, DEAE cellulose ion exchange chromatography with Nacl and pH gradient aqueous solution, and preparative polyacrylamide gel electrophoresis.