Abstract
Bioethanol is a liquid chemical produced from sugar-, starch-or lignocellulosic-based biomass through fermentation by ethanol-producing microbes. Ethanol-producing yeast generally has limited tolerance to ethanol and has limitation to high temperatures above 40°C. High-temperature tolerant yeast is required because it potentially reduces the risk of contamination and it also reduces the cost of the cooling process. This study aims to determine ethanol-producing yeasts that have tolerance to ethanol and high temperatures from local fermented food products. This study uses a descriptive method conducted in three stages. Isolation and selection of yeast were performed from 18 local fermented foods in Indonesia. Temperature and ethanol tolerance of selected yeast were performed by using a spot test method. The ethanol content was tested using Gas Chromatography (GC). The results exhibited that isolate F08b had the highest tolerance to ethanol and temperature. The isolate was able to grow up to a temperature of 50°C and a concentration of 18% ethanol. Meanwhile, isolate F10 was able to produce the highest ethanol concentration at 3.37% (v/v) in 48th-hour fermentation.
Highlights
Bioethanol is the metabolite produced from glucose-containing materials through fermentation by beneficial microorganisms such as bacteria and yeast
Conventional yeast which acts as a bioethanol producer has a limited tolerance to temperatures above 40oC and has a limited tolerance to the level of ethanol concentration above 20% [1]
The media used in this study were Yeast extract Peptone Glucose Agar (YPGA) and Yeast extract Peptone Glucose Broth (YPGB)
Summary
Bioethanol is the metabolite produced from glucose-containing materials through fermentation by beneficial microorganisms such as bacteria and yeast. Conventional yeast which acts as a bioethanol producer has a limited tolerance to temperatures above 40oC and has a limited tolerance to the level of ethanol concentration above 20% [1]. Yeast will unable to grow during the fermentation process as well as unable to produce bioethanol. The ethanol-producing yeast that has resistant to heat and high ethanol concentration is necessary for the development of industrial biofuel. The thermotolerant yeasts are able to survive at high temperatures of 40oC or above. While yeast can be categorized into ethanol tolerant depend on their ability to survive in an environment that has an ethanol concentration of 10% or more [4]
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