Abstract
Purpose: The Copaifera species (Leguminoseae) are popularly known as “copaiba” or “copaiva” and are grown in the states of Amazonas, Para, and Ceara in Northern Brazil. The oleoresins obtained from the trunk of these trees have been extensively used in folk medicine and are known by their anti-inflammatory, antioxidant, antimicrobial properties and antitumor activity against various kinds of tumor cells. The present work aims to isolate the main metabolites of the oleoresin of Copaifera paupers and to evaluate its in vitro cytotoxic potential against tumor cell lines. Methods: The isolation processes and purification of the oleoresin diterpenes were conducted using various chromatographic techniques, such as vacuum liquid chromatography (VLC), classical column chromatography (CC), thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC). The chemical structures of the isolated compounds are being identified using spectrometric methods such as nuclear magnetic resonance (NMR). Results and conclusions: Up to now, the isolation procedure gave fractions OCP2.4.7, OCP 2.4.5.7, OCP 2.4.8.2, OCP 2.5.11, OCP 2.5.5 e OCP 2.6 which are in the structural elucidation phase. The evaluation of the cytotoxicity of Copaifera paupera oilresin was already performed for MCF10 (normal cell line) through the XTT method. The IC 50 value was 109,0 ± 1,10 µg.mL -1 .
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