Abstract
Periplocosides, which are insecticidal compounds isolated from the root bark of Periploca sepium Bunge, can affect the digestive system of insects. However, the mechanism though which periplocosides induces a series of symptoms remains unknown. In this study, affinity chromatography was conducted by coupling periplocoside E-semi-succinic acid ester with epoxy amino hexyl (EAH) sepharose 4B. Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) was performed to analyze the fraction eluted by periplocoside E. Eight binding proteins (luciferin 4-monooxygenase, aminopeptidase N, aminopeptidase N3, nicotinamide adenine dinucleotide health (NADH) dehydrogenase subunit 5, phosphatidylinositol 3-phosphate 3-phosphatase myotubularin, actin, uncharacterized family 31 glucosidase KIAA1161, and 2OG-Fe(2) oxygenase superfamily protein) were obtained and identified through liquid chromatography/quadrupole-time of flight-mass spectrometry (LC/Q-TOF-MS) analysis of the midgut epithelium cells of Mythimna separata larvae. Aminopeptidase N and N3 are potential putative targets of periplocosides. This study establishes the foundation for further research on the mechanism of action and target localization of periplocosides in agricultural pests.
Highlights
Global pesticide production should be highly effective, confer low toxicity and high safety coefficient, and exhibit satisfactory selectivity and environmental compatibility
The use of biological active ingredients [3] or action targets [4] as guides is an important aspect in research and development of botanical pesticides
Our preliminary results revealed that the brush-border membrane vesicles (BBMVs) from the midgut epithelium cells of M. separata
Summary
Global pesticide production should be highly effective, confer low toxicity and high safety coefficient, and exhibit satisfactory selectivity and environmental compatibility. P. sepium secondary metabolites contain periplocosides, which are pregnane glycosides with evident insecticidal activity against several insect species [7] These compounds possess a special mode of action that involves. These possess a special of insecticidal action that involves metabolites contain periplocosides, whichcompounds are pregnane glycosides withmode evident activity obviousseveral swelling of the abdomen, and,compounds far, nopossess other modes of mode insecticidal action been against insect species [7] These a special of action thathave involves recordedswelling [8]. M. larvae separata are the initial action site; onebinding or moresites binding sites mayof periplocosides in separata larval midgut cells remain unclear. Periplocoside E has relatively low insecticidal activity or is inactive high consumption of experimental to determine theselected binding protein of periplocosides compared with. Through affinity chromatography to elucidate theofmode of action of the compound
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