Isolation of the ɛ-caprolactam denitrifying bacteria from a wastewater treatment system manufactured with acrylonitrile–butadiene–styrene resin

Abstract

ɛ-Caprolactam has high COD and toxicity, so its discharge to natural water and soil systems may lead to an adverse environmental effect on water quality, endangering public health and welfare. This investigation attempts to isolate ɛ-caprolactam denitrifying bacteria from a wastewater treatment system manufactured with acrylonitrile–butadiene–styrene (ABS) resin. The goal is to elucidate the effectiveness of isolated pure strain and ABS mixed strains in treating ɛ-caprolactam from synthetic wastewater. The results reveal that Paracoccus versutus MDC-3 was isolated from the wastewater treatment system manufactured with ABS resin. The ABS mixed strains and P. versutus MDC-3 can consume up to 1539 mg/l ɛ-caprolactam to denitrify from synthetic wastewater. Complete ɛ-caprolactam removal depended on the supply of sufficient electron acceptors (nitrate). Strain P. versutus MDC-3, Hyphomicrobium sp. HM, Methylosinus pucelana and Magnetospirillum sp. CC-26 are related closely, according to the phylogenetic analyses of 16S rDNA sequences.