Isolation of tannase-producing Enterobacter ludwigii GRT-1 from the rumen of migratory goats

Abstract

We report for the first time isolation and characterization of a tannase-producing anaerobe from the rumen of migratory goats browsing on forage containing the anti-nutritional hydrolysable tannin. A total of sixteen bacterial isolates were isolated. The isolates were capable of degrading tannic acid to gallic acid and pyrogallol. One of the isolates due to its unique tolerance up to a level of 3.5% (w/v) of tannic acid, and production of tannase was further investigated and identified as Enterobacter ludwigii GRT-1, based on 16S rRNA gene sequencing.