Abstract
SINCE the discovery of ‘synaptic vesicles’ as a specific component of synapses1,2 it was suggested that they could be associated with the storage and possibly with the synthesis of transmitter substances. This concept was supported by degeneration experiments3 and specially by the stimulation of some cholinergic terminals4, which suggested that within the ending a balance exists between the formation and release of synaptic vesicles. However, the isolation of such a sub-cellular component remained as the most direct approach to the elucidation of the physiological significance and chemical nature of these vesicles.
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