Isolation of Soil Bacteria with Inhibitory Activity to Pseudomonas Solanacearum

Abstract

Recent reports from several investigators throughout the world have indicated the potential use of prokaryotes to control plant pathogenic bacteria. We report here an effective method for the isolation and detection of soil bacteria with inhibitory activity to Pseudomonas solanacearum.True seeds of Solanum tuberosum were grown under greenhouse conditions in flats containing a non-sterile garden soil. Each flat was root inoculated with P. solanacearum. Flats were incubated at 28C in an environmental chamber for up to 21 d. After this period, surviving plants were used for isolations from inner tissues.Bacteria were selected from TZC isolation plates and exposed to chloroform fumes for 2 h. A second layer of soft agar containing a suspension of P. solanacearum was placed on top of the chloroform TZC agar plates and incubated for 48 h at 28C. Inhibitions were recorded and isolates with antagonistic activity were kept for identification and further studies. Almost all surviving plants from inoculated flats contained bacteria in their vascular tissues and some of them with strong antagonistic activity to P. solanacearum.KeywordsBiological ControlSolanum TuberosumSoft AgarBacterial WiltPlant Pathogenic BacteriumThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.