Abstract
Here the feasibility is demonstrated that by combining Surface Plasmon Resonance Imaging (SPRi) and self-sorting microwell technology product secretion of individual cells can be monitored. Additionally isolation of the selected cells can be performed by punching the cells from the microwells using coordinates of the positions of microwells obtained with SPRi. Cells of interest can be retrieved sterile from the microwell array for further cultivation.
Highlights
A range of biopharmaceuticals, therapeutic recombinant monoclonal antibodies [1], are currently produced in high amounts for therapeutic applications
SPR has been considered as a label free method for mainly protein quantification [7e9] only recently, a study reported the quantification of proteins derived from whole intact single cells using SPR
Stojanovic et al demonstrated the feasibility to monitor and quantify the amount of product excreted by whole intact single cells on top of an SPR sensor surface using real-time label free SPR imaging (SPRi) [10]
Summary
A range of biopharmaceuticals, therapeutic recombinant monoclonal antibodies (mAbs) [1], are currently produced in high amounts for therapeutic applications. Stojanovic et al demonstrated the feasibility to monitor and quantify the amount of product excreted by whole intact single cells on top of an SPR sensor surface using real-time label free SPR imaging (SPRi) [10]. After monitoring and determining individual cells in the SPR image, high producer cells could not be retrieved from the sensor surface.
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