Abstract
Foodborne pathogens are the main threat and cause of food poisoning. The majority of food infections have been related to the biofilm formation of foodborne pathogens in the food industry. Shewanella putrefaciens (KX355803, GRD 03), a Gram-negative pathogen isolated from mackerel fish, was identified and recognized as a food spoilage bacterium and a strong biofilm producer. The adhesion or attachment ability of Shewanella putrefaciens was determined on steel, plastic, glass, PVC and wood. NB (Nutrient broth), LB (Luria-Bertani broth), TSB (Tryptic soy broth) and BHI (Brain heart infusion broth) were enriched with glucose and shows optimum for bacterial adhesion. In the microtiter plate method (MTP), the strong attachment was observed at 48 and 72 hours of incubation and significant differences were obtained at p < 0.05. As the incubation period increases, the OD value (Optical density) of samples also increase. Biofilm formation is the major cause cross-contamination, and shows resistance to certain disinfectants, which leads to environmental stress tolerance. This study suggested with optimum biofilm production of isolate from fish by using glucose enriched media on different substrates, also comparing different growth media provide a detailed idea about biofilm-forming ability at different incubation time intervals.
Highlights
Shewanella putrefaciens play a predominant role in food spoilage processes, mainly correlated with spoilage of frozen fish and meat products.[1,2] This pathogen has a potential to live in fresh water habitation.[1]
This study suggested with optimum biofilm production of isolate from fish by using glucose enriched media on different substrates, comparing different growth media provide a detailed idea about biofilm forming ability at different incubation time intervals
Bacterial cells communicate with each other via some chemically produced signaling molecules known as Autoinducers (AI), and the process stated as Quorum sensing (QS)
Summary
Shewanella putrefaciens play a predominant role in food spoilage processes, mainly correlated with spoilage of frozen fish and meat products.[1,2] This pathogen has a potential to live in fresh water habitation.[1]. Biofilm process required different stages, involved, attachment of bacterial cells, maturation and dispersion.[5] To cope with this bacterial settlement in biofilm, a serious hygiene approach is mandatory, which comprises disinfection and proper cleaning. Nowadays, these biofilm producers have the potentiality to fight against such disinfectants.[6,5] Briedier et al.[7] discussed about some foodborne pathogens mainly involved in biofilm production and adhesion on different substrates in the food industry.[7] this present study targets the isolation and identification of foodborne pathogen Shewanella putrefaciens from fish sample and its biofilm formation capacity on different substrates
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