Isolation of Rhizosphere and Phyllosphere Bacteria from Cereal Samples

Abstract

This study presents the isolation of rhizosphere and phyllosphere bacteria from cereal samples, highlighting their importance in agricultural ecosystems. Employing surface sterilization techniques, selective media and molecular analysis, diverse bacterial communities were identified on the root and leaf surfaces of cereal crops, encompassing taxa from the Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes phyla. A total of 40 rhizosphere and phyllosphere bacteria were isolated from different cereal crops This research contributes to our understanding of microbial dynamics within cereal crop ecosystems and informs strategies for optimizing agricultural practices to meet the challenges of global food security and environmental sustainability.