Abstract
AbstractCloned resistance (R) genes from a broad range of plant species are known to share similarities in DNA sequences and structural motifs. Oligonucleotide primers, designed to conserved regions of nucleotide binding site (NBS) motifs within previously cloned pathogen resistance genes, were used to amplify resistance gene analogues (RGAs) from dogwood. Twenty eight unique dogwood RGA sequences were identified and subdivided into 11 groups on the basis of nucleic acid sequence‐identity of approximately 70% or greater. Phylogenetic analysis of the predicted amino acid sequences grouped the RGAs into three distinct classes from which several subgroups were delineated based on nucleic acid sequences. Gene database searches with the consensus protein sequences of each of the three classes and respective subgroups of dogwood RGAs revealed their conserved NBS domains and homology to RGAs and known resistance genes from a variety of plant genera. Given the complete lack of knowledge regarding molecular organization of R genes in dogwood, the cloned RGAs described here may be useful as probes to map, characterize, and manipulate R genes of the dogwood genome.
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