Abstract
In order to identify promoter sequences related to the differentiation of nematode feeding sites, a collection ofArabidopsis thalianaplants transformed with a promoterlessgus-construct was screened by infection with the root-parasitic nematode Heterodera schachtii.Line 884 showedgusactivity in nematode feeding structures (NFS), leaf hydathodes, and stipules. The most frequentgusactivity in NFS was detected 7 days after infection. DNA gel blot analysis indicated a single copy of the T-DNA in line 884. Using inverse PCR, a 963 bp fragment 5′ to the T-DNA was isolated and used to screen a genomic library ofA. thaliana.A 4.2 kb fragment containing the insertion site was isolated. Six fusion constructs consisting of different sequences of the isolated genomic clone and thegusgene was transformed intoArabidopsisplants. In this way it was possible to identify regulatory regions which are essential togusexpression in NFS.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
