Abstract
AbstractPorcine epidemic diarrhea virus (PEDV) infects all ages of pigs and causes porcine epidemic diarrhea (PED) (Jung et al., Virus Res 286: 198045, 2020). Clinical signs include acute diarrhea, vomiting, anorexia, weight loss, dehydration, and depression. Disease is more severe in young pigs than adult pigs. Especially in neonatal piglets, PEDV causes high mortality rates (up to 100%). To date, PEDV circulates in Asia, Europe, and Americans, resulting in huge losses in the pork industry. Although nucleic acid detection and sequence analysis are popular in disease diagnosis, the isolation of infectious PEDV from clinical samples is still irreplaceable for the study of virus replication, the evaluation of disinfectants, and the development of antiviral drugs and vaccines. In this chapter, detailed materials and methods for the cell culture isolation of PEDV variants from clinical samples are provided. Clinical sample types for the successful isolation of PEDV will be discussed.Key wordsPorcine epidemic diarrhea (PED)PED virus (PEDV)IsolationVirusPigSwineCell cultureCoronavirusAlphacoronavirusEnteric disease
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