Abstract
Dendrobium officinale stem is rich in polysaccharides, which play a great role in the medicinal effects of this plant. However, little was known about the polysaccharides from Dendrobium officinale leaves. Two kinds of polysaccharides in the leaves, DLP-1 and DLP-2, were obtained by hot water extraction, alcohol sedimentation and chromatographic separation (DEAE-52 cellulose column and Sephadex G-100 column). The average molecular weights were determined as 28,342 Da and 41,143 Da, respectively. Monosaccharide compositions were analyzed using gas chromatography–mass spectrometer. DLP-1 was composed of d-(+)-galactose, dl-arabinose, and l-(+)-rhamnose with a molar ratio of 3.21:1.11:0.23, and traces of d-xylose, d-glucose, and d-(+)-mannose. DLP-2 was consisted of d-glucose and d-(+)-galactose with a molar ratio of 3.23:1.02, and traces of d-xylose, dl-arabinose. Then, we established inflammatory cell model by LPS acting THP-1 cells to investigate the anti-inflammatory effects of DLP-1 and DLP-2. The results indicated that DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) were effective in protecting THP-1 cells from LPS-stimulated cytotoxicity, as well as inhibiting reactive oxygen species formation. In addition, both DLP-1 (5 μg/mL) and DLP-2 (50 μg/mL) significantly suppressed toll-like receptor-4 (TLR-4), myeloid differentiation factor (MyD88) and tumour necrosis factor receptor-associated factor-6 (TRAF-6) mRNA and protein expression in LPS-stimulated THP-1 cells.
Highlights
Dendrobium officinale Kimura et Migo belongs to Dendrobium Sw., Orchidaceae and widely distributes in the tropical and subtropical areas [1]
The results showed that the average molecular weight of DLP-1 and DLP-2 were 28,342 Da and 41,143 Da, respectively (Table 1)
DLP-2 was consisted of d-glucose and d-(+)-galactose in a mole ratio of 3.23:1.02, and traces of d-xylose and dl-arabinose (Table 2)
Summary
Dendrobium officinale Kimura et Migo belongs to Dendrobium Sw., Orchidaceae and widely distributes in the tropical and subtropical areas [1]. Its stem is usually processed into one of the traditional Chinese medicine named “Tiepishihu” after twisted into a spiral while baking and used as a tonic for more than 2000 years due to its exceptional effect [3–6]. It could be either chewed directly or. The current research on Dendrobium officinale focuses on stems, and surprisingly little was known about leaves until now. The stems and leaves originate from the same plant, so Dendrobium officinale leaves should have a great range of potential utilities and a prospect of development in food, medical and health care. Dendrobium officinale leaves exhibited good auxiliary therapeutic effect on hypertension, hyperglycemia, hyperlipidemia and other similar symptoms as well as promoting health when serving as tea [8, 9], and there was a research indicated that Dendrobium officinale
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