Abstract
ABSTRACTSimple, fast and low cost method for isolation of plasmid DNA, affordable for any laboratory is presented. The method is based on the specific adsorption of double stranded (ds) DNA on glass fibers in the presence of high concentration of guanidine hydrochloride (GnHCl) and elution of the retained DNA with ordinary buffers. Among the various types of glass filters and other silicate materials tested, the most appropriate for isolation of plasmid DNA was found to be the Schleicher & Schuell glass filters GF92. The best retention of dsDNA on these filters was achieved in 1.5 M GnHCl, pH 4.0. The plasmid DNA preparations obtained by this method contained mainly supercoiled plasmid DNA endowed with a high transformation activity. Besides for transformation, this DNA can be used also for restriction endonuclease mapping and sequencing.
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