Abstract
Pigeonpox virus was isolated from severe cases of avipoxvirus infection affecting 64 pigeons in Ba'aquba of Diyala governarate. The virus grew well on chorioallantoic membrane of chick embryos of 11-12 days old, and produced typical pock lesions. Histopathological sections of infected tissue samples revealed typical pox lesions. The virus was identified as Pigeonpox virus using of specific hyper immune serum and indirect immuno-flourescent and indirect immuno-peroxidase tests. The virus agglutinated RBCs of pigeon, fowl, turkey and duck. Experimental infection in pigeons produced moderate infection as compared to the diseased birds, while in chicken the virus produced mild infection.
Highlights
Severe cases of pigeon poxvirus infection were admitted to the private Veterinary clinic in Baaquba city of Diyala
Some samples were kept at 10% buffered formalin for histopathological and histo-immunological tests
Embryonated hen eggs 1112 days old obtained from local hatcharies were used for virus isolation and 0.1 ml of processed sample was inoculated on chorioallantoic membrane (CAM) [11]
Summary
The virus grew well on chorioallantoic membrane of chick embryos of 11-12 days old, and produced typical pock lesions. Histopathological sections of infected tissue samples revealed typical pox lesions. The virus was identified as Pigeonpox virus using of specific hyper immune serum and indirect immuno-flourescent and indirect immunoperoxidase tests. The virus agglutinated RBCs of pigeon, fowl, turkey and duck. Experimental infection in pigeons produced moderate infection as compared to the diseased birds, while in chicken the virus produced mild infection
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